BIOC2000 PCR simulators

In this simulator you will see the sequence of the GFP gene that we want to clone into a plasmid vector.

The coding sequence is highlighted with asterisks, and a large part from the center of the coding sequence has been removed to fit better on screen. The second simulator on this page will load with a full size version of the gene.

Your task is to design primers that will amplify the coding sequence so that it can be insterted into the pGEX-2T plasmid vector.

This version loads with the complete GFP gene sequence you will use in snapgene. You can try out your finished primers here to see how they will really work.